A Review Article on Euphorbia hirta uses and pharmacological activities

 

Mr. P.M. Kale

Lecturer, SATS Diploma in Pharmacy College, Miraj-416414

 

ABSTRACT:

The herbal medicines are oldest remedies known to mankind. India is known worldwide for its Ayurvedic treatment. Euphorbia hirta is often used traditionally for female disorders, respiratory ailments (cough, coryza, bronchitis, and asthma), and worm infestations in children, dysentery, jaundice, pimples, gonorrhea, digestive problems, and tumors. E. hirta is native to Central America. It was introduced into Southeast Asia a long time ago and has since spread throughout. It is a very common herb in the pan-tropic and partly subtropic areas worldwide, including China, India, Philippines, Australia, Africa, Malaysia, and so on. It is reported to contain alkanes, triterpenes, phytosterols, tannins, polyphenols, and flavanoids. Recently, modern pharmacological investigations showed that E. hirta and its active components possessed wide pharmacological actions, such as anti-inflammatory, antifungal, anti-bacterial, antidiarrheal, sedative, anxiolytic, analgesic, antipyretic, antioxidant, antiasthmatic, antitumor, antimalarial, larvicidal, diuretic, and increases electrolytes, among others. This review describes the medicinal properties, chemical constituents, and other important aspects of Euphorbia hirta.

 

 

 

INTRODUCTION:

The indigenous system of medicine, namely, Ayurvedic, Siddha, and Unani, has been in existence for several centuries. Some drugs from Ayurveda approaches modern diseases, have already reached the market place.[1] Though the Synthetic drugs are effective in controlling different diseases but these synthetic drugs are out of reach of millions of people. It is estimated that around 70,000 plant species have been used for medicinal purposes. The herbs provide the starting material for the synthesis of conventional drugs. Medicinal plants have curative actions due to the presence of complex chemical constituents. India recognizes more than 2500 plant species having medicinal value, Sri Lanka around 1400, and Nepal around 700.[2]

 

This review intends to provide an overview of the chemical constituents, uses and pharmacological actions of Euphorbia hirta.

 

General Information:

The largest genus of family Euphorbiaceae is Euphorbia with about 1600 species. It is characterized by the presence of white milky latex which is more or less toxic. Latices of E. ingens, E. mey, E. tirucalli, and E. triangularis are possible sources of rubber.[3] This group of plants has been a subject of intense phytochemical examination and isolated compounds which include:- flavanoids, triterpenoids, alkanes, amino acids, and alkaloids.[1] E. ipecacuanha is known as wild ipecac; E. antiquorum is known as Tridhara; E. lathyrus is known as caper spurge; and E. thymifolia is known as Laghududhika.[2]There are many other species of Euphorbia which are used in traditional medicines. All species of Euphorbia exudes a milky juice when broken, which is more or less poisonous and used as an ingredient in arrow poisons. E. hirta possesses antibacterial, anthelmintic, antiasthmatic, sedative, antispasmodic, antifertility, antifungal, and antimalarial properties.[1]

 

Geographical Distribution:

E. hirta is distributed throughout the hotter parts of India, Africa , Central America and Australia, often found in waste places along the roadsides.[6]

 

Plant Description:

E. hirta Linn. Syn; E. pilulifera Linn. Chamaesyce pilulifera Linn.[5] Family: Euphorbiaceae

 

Vernacular Names:

 

 

MORPHOLOGY:

E. hirta belongs to the plant family Euphorbiaceae and genus Euphorbia. It is a slender- stemmed, annual hairy plant with many branches from the base to top, spreading upto 40 cm in height, reddish or purplish in color. Leaves are opposite, elliptic - oblong to oblong- lanceolate, acute or subacute, dark green above, pale beneath, 1- 2.5 cm long, blotched with purple in the middle, and toothed at the edge. The fruits are yellow, three- celled, hairy, keeled capsules, 1-2 mm in diameter, containing three brown, four-sided, angular, wrinkled seeds.[1–4]

 

ETHNOPHARMACOLOGY:

E. hirta is used in the treatment of gastrointestinal disorders (diarrhea, dysentery, intestinal parasitosis, etc.), bronchial and respiratory diseases (asthma, bronchitis, hay fever, etc.), and in conjunctivitis. Hypotensive and tonic properties are also reported in E. hirta. The aqueous extract exhibits anxiolytic, analgesic, antipyretic, and anti-inflammatory activities. The stem sap is used in the treatment of eyelid styes and a leaf poultice is used on swelling and boils.[3]

 

Extracts of E. hirta have been found to show anticancer activity. The aqueous extract of the herb strongly reduced the release of prostaglandins I2, E2 and, D2 [3] The aqueous extract also inhibits aflatoxin contamination in rice, wheat, maize, and mustard crops.[7] Methanolic extract of leaves have antifungal and antibacterial activities.

 

The leaves pounded with turmeric and coconut oil are warmed and rubbed on itchy soles. The latex of E. hirta is applied on lower eyelids, like surma to cure eye sores. The root exudate exhibits nematicidal activity against juveniles of meloidogyne incognita.[3]

 

Decoction of dry herbs is used for skin diseases. Decoction of fresh herbs is used as gargle for the treatment of thrush. Root decoction is also beneficial for nursing mothers deficient in milk. Roots are also used for snake bites.[1] The polyphenolic extract of E. hirta has antiamoebic[8] and antispasmodic activity.[9] Quercitrin, a flavanoid glycoside, isolated from the herb showed an antidiarrheal activity.[10–11] It is reported to have a relaxation effect on respiration.[12] The alcoholic extract of whole plant shows hypoglycemic activity in rats.[6] It has a sedative effect on the genitor-urinary tract.[4]

 

CHEMICAL CONSTITUENTS:

E. hirta has been studied by various workers and a number of active constituents have been isolated. Afzelin (I), quercitrin (II), and myricitrin (III) have been isolated from the methanolic extract of E. hirta.[13] The chemical investigation of E. hirta has led to the isolation of rutin (IV), quercitin (V), euphorbin-A (VI), euphorbin-B (VII), euphorbin-C (VIII), euphorbin-D (IX), 2,4,6-tri-O-galloyl-β-D-glucose, 1,3,4,6-tetra-O-galloyl-β-D-glucose, kaempferol, gallic acid, and protocatechuic acid.[14–15] E. hirta also contains β-amyrin, 24-methylenecycloartenol, β-sitosterol, heptacosane, nnonacosane,[1] shikmic acid, tinyatoxin, choline, camphol, and quercitol derivatives containing rhamnose and chtolphenolic acid.

 

Fig: The Structure of main compounds from E.Hirta [1]

 

PHARMACOLOGICAL ACTIVITIES:

Antibacterial activity:

The ethanolic extract of E. hirta inhibited the growth of the Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Bacillus subtili [16] and aqueous and chloroform leaf extracts of E. hirta possess an antibacterial activity against Klebsiella pneumonia. The extract is noncytotoxic and antibacterial.[17]

 

Antimalarial activity:

The bioassay-guided fractionation of the methanolic extract of aerial parts of E. hirta, monitored against P. falciparum parasites, yielded a main active chromatographic fraction showing 90% growth inhibition of P. falciparum at a concentration of 5 μg/ml.[13]

 

Anti-inflammatory activity:

The n-hexane extract of aerial parts of E. hirta showed anti-inflammatory effects in the model of phorbol acetate-induced ear inflammation in mice. It exhibited a dose-dependent effect.[18,19]

 

 

Galactogenic activity

The powdered E. hirta showed a galactogenic activity in guinea pigs before puberty by increasing the development of the mammary glands and induction of secretion.[20]

 

Antiasthmatic activity:

E. hirta is reported to have an antiasthmatic activity due to the relaxation effect on the bronchial tubes and a depressant action on respiration.[12]

 

Effect on urine output and electrolytes:

Ethanolic and aqueous leaf extracts of E. hirta significantly induced diuresis in rats. The diuretic effect of the ethanol extract was significant at 6 h (for 100 mg/kg) and at 24 h (for 50 mg/kg). The water extract induced a significant increase in urine Na+, K+ and HCO3- loss. The ethanol extract (100 mg/ml) caused a significant decrease in the K+ loss whereas the water extract increased its excretion. The HCO3- urine output following the injection of both extracts was tremendously enhanced.[21]

 

Antidiarrheal activity:

The antidiarrheal effect of the herb decoction was studied in mice. It demonstrated an activity in models of diarrhea induced by castor oil, arachidonic acid, and prostaglandin E2.[10] Quercitrin, a flavanoid glycoside isolated from E. hirta, showed an antidiarrheal activity, at a dose of 50 mg/kg, against castor oil and prostaglandin E2-induced diarrhea in mice.[11]

 

Antioxidant activity:

The aqueous extract of E. hirta L. showed an antioxidant effect and a free radical scavenging activity in various in vitro models like total antioxidant and total ferric reducing power determination, assay for free radical-scavenging activity using ABTS, DPPH, and hydroxyl radical scavenging assays. It showed maximum antioxidants and free radical scavenging activities, at 0.25 mg/ml. The free radical scavenging effect on DPPH and hydroxyl was found as 68.80 ± 5.21 and 73.36 ± 5.21%, respectively.[22]

 

Antifertility activity:

E. hirta at a dose of 50 mg/kg reduced the sperm motility and density of cauda epididymal and testis sperm suspension significantly, leading to 100% infertility.[23]

 

Antiamoebic activity:

The polyphenolic extract of E. hirta inhibited the growth of Entamoeba histolytica with a minimum active concentration of less than 10 μg/ml.[8]

 

Antifungal activity:

An ethanolic extract of E. hirta showed an antifungal activity against plant pathogens Colletotrichum capsici, Fusarium pallidoroseum, Botryodiplodia theobromae, Phomopsis caricae-papayae, and Aspergillus niger using the paper disc diffusion technique. [24]

 

Immunomodulatory activity:

In 2001, the whole plant of E. hirta has been reported to possess 45% immunomodulation activity through the inhibition of NO production which has been proven through macrophage activity testing, carbon clearance test, and mast cell de-granulation assay.

 

Other use [26]:

·        It is used for relieving toothache, severe headache, rheumatism and colic.

·        The extract of the plant is used as an antidote for snakebites and scorpion stings.

·        The latex of the plant is used to remove thorns from the skin.

·        Uro-genital diseases like kidney stones, menstrual problems and venereal diseases are relieved using this plant.

·         Crushed leaves with water to make paste and applied externally over the wounds and ulcers.

·        Apply leaf paste over swelling.

·        Latex of plant helps to heal warts fastly.

·        For leucohorrhoea take half teaspoon leaf paste.

·        Leaves in the form of chutney in diet act as laxative.

 

SAFETY PRECAUTIONS AND SIDE EFFECTS IN USING EUPHORBIA HIRTA:

·        Euphorbia hirta should never be used during pregnancy. Over dosage will lead to complications. This herb should be used only under the guidance of an authorized practitioner. [26]

·        When taken by mouth, it can cause some side effects such as nausea and vomiting.

·        Don’t touch the fresh herb. It can cause skin irritation or allergic reactions.

·        Special Precautions and Warnings:

·        Pregnancy and breast-feeding: It is possibly unsafe to take Euphorbia by mouth if you are pregnant. There is some evidence that it might cause the uterus to contract, and this could cause a miscarriage.

·        Stomach or intestinal problems: Euphorbia can irritate the stomach and intestines. Don’t use it if you have a stomach or intestinal disorder.[27]

 

SUMMARY:

In the present review, we have made an attempt to provide the mophological, phytochemical, ethno pharmacological and pharmacological information on E. hirta, a herb used traditionally for medicinal purposes. The literature survey revealed that E. hirta contains afzelin, quercitrin, myricitrin, rutin, gallic acid. This herb shows antibacterial, anti-inflammatory, antimalarial, galactogenic, antiasthmatic, antidiarrheal, anticancer, antioxidant, antiferlity, antiamoebic, and antifungal activities. Further research is going on to find out more activities in constituents of E. hirta. There are many other traditional uses of E. hirta in Ayurveda which serves as the basis for further studies. This review will definitely help the researchers to know its different properties.

 

REFERENCES:

1.       Lanhers et al, 1990; Lanhers et al, 1991; Johnson et al, 1999; Euphorbia hirta L. Available: http:// florabase.dec.wa.gov.au/browse/profile.php/4629) Williamson EM. China: Churchill Livingstone; 2002. Major Herbs of Ayurveda.

2        Prajapati ND, Purohit SS, Sharma AK, Kumar T. Jodhpur, India: Agarbios; 2003. Handbook of Medicinal Plants.

3        Vol. 3. New Delhi: Council of Industrial and Scientific Research; 2005. The Wealth of India (Raw material)

4        Kirtikar KR, Basu BD. Dehradun, India: Oriental Enterprises; 2003. Indian medicinal plants with illustrations.

5        Nadkarni KM. 4th. Bombay, India: Popular Prakashan; 1976. Indian Materia Medica.

6        Sood SK, Bhardwaj R, Lakhanpal TN. India: Scientific Publishers; 2005. Ethnic Indian Plants in cure of diabetes.

7        Singh P, Sinha KK. Inhibition of aflatoxin production on some agricultural commodities through aqueous plant extracts. J Indian Bot Soc. 1986;65:30–2.

8        Tona L, Kambu K, Ngimbi N, Mesia K, Penge O, Lusakibanza M, et al. Antiamoebic and spasmolytic activities of extracts from some Antidiarrhoeal traditional preparations used in Kinshasa and Congo. Phytomedicine. 2000;7:31–8. [PubMed]

9        Gnecco S, Perez C, Bittner M, Silva YM. Distribution pattern of n-alkanes in Chilean species from the Euphorbiaceae family. Bol Soc Chil Quim. 1996;41:229–33.

10     Galvez J, Zarzuelo A, Crespo ME, Lorente MD, Ocete MA, Jimenez J. Antidiarrheal activity of Euphorbia hirta extract and isolation of an active flavanoid constituent. Planta Med. 1993;59:333–36. [PubMed]

11     Galvez J, Crespo ME, Jimenez J, Suarez A, Zarzuelo A. Antidiarrheic activity of quercitrin in mice and rats. J Pharm Pharmacol. 1993;45:157. [PubMed]

12     Chopra RN, Chopra IC, Handa KL, Kapur LD. Calcutta, India: Academic Publishers; 1994. Indigenous drugs of India.

13     Liu Y, Murakami N, Ji H, Abreu Pedro, Zhang S. Antimalarial flavonol glycosides from Euphorbia hirta. Pharm Biol. 2007;45:278–81.

14     Rastogi RP, Mehrotra BN. 3rd. Lucknow, India: Central Drug Research Institute; 2002. Compendium of Indian Medicinal Plants, 3rd Vol.

15     Rastogi RP, Mehrotra BN. 4th. Lucknow, India: Central Drug Research Institute; 2002. Compendium of Indian Medicinal Plants.

16     Ogbulie JN, Ogueke CC, Okoli IC, Anyanwu BN. Antibacterial activities and toxicological potentials of crude ethanolic extracts of Euphorbia hirta. Afr J Biotechnol. 2007;6:1544–8.

17     Suresh K, Deepa P, Harisaranraj R, Vaira Achudhan V. Antimicrobial and phytochemical investigation of the leaves of Carica papaya L., Cynodon dactylon (L.) Pers., Euphorbia hirta L., Melia azedarach L. and Psidium guajava L. Ethnobotanical Leaflets. 2008;12:1184–9.

18     Martinez V, Mariano A, Teresa OR, Lazcano ME, Bye R. Anti-inflammatory active compounds from the n-hexane extract of Euphorbia hirta. Rev Soc Quim Méx. 1999;43:103–5.

19     Lanhers MC, Fleurentin J, Dorfman P, Mortier F, Pelt JM. Analgesic, antipyretic and antiinflammatory properties of Euphorbia hirta. Planta Med. 1991;57:225–31. [PubMed]

20     Blanc P, Bertrand P, de Saqui-Sannes G, Lescure R. Galactogenic properties of plants of the African flora: Sersalisia djalonensis and Euphorbia hirta. Ann Biol Clin (Paris) 1963;21:829.

21     Johnson PB, Abdurahman EM, Tiam EA, Abdu-Aguye I, Hussaini IM. Euphorbia hirta leaf extracts increase urine output and electrolytes in rats. J Ethnopharmacol. 1999; 65:63–9. [PubMed]

22     Sharma NK, Dey S, Prasad R. In vitro antioxidant potential evaluation of Euphorbia hirta L. Pharmacologyonline. 2007;1:91–8.

23     Mathur A, Dixit VP, Dobal MP. Antifertility plant product: Euphorbia hirta in males. Proceedings of the International Symposium on Male Contraception: Present and Future. 1995

24     Mohamed S, Saka S, EL-Sharkawy SH, Ali AM, Muid S. Antimycotic screening of 58 Malaysian plants against plant pathogens. Pestic Sci. 1996;47:259–64.

25     Euphorbia hirta: Its chemistry, traditional and medicinal uses, and pharmacological activities Sunil Kumar, Rashmi Malhotra, and Dinesh Kumar

26     http://www.alwaysayurveda.com/euphorbia-hirta

27     http://www.webmd.com/vitamins-supplements/ingredientmono-636-euphorbia.aspx?activeingredientid=636andactiveingredientname=euphorbia

 

 

 

Received on 12.07.2016       Accepted on 30.08.2016     

© Asian Pharma Press All Right Reserved